Diagnostic Kits

ISCA Director Dr Christopher Thornton invented the Aspergillus lateral-flow device, a point-of-care test for rapid detection of invasive pulmonary aspergillosis, which uses the patented monoclonal antibody JF5. The CE marked test is due for launch in 2017 and can be purchased from OLM Diagnostics (info@olmdiagnostics.com). 

For further information on the Aspergillus LFD and testing of bronchoalveolar lavage (BAL) and serum samples see the educational video below.

Publications describing the Aspergillus lateral-flow device   

Thornton CR. (2008). Development of an Immunochromatographic Lateral-Flow Device for Rapid Serodiagnosis of Invasive Aspergillosis. Clinical and Vaccine Immunology 15: 1095-1105.

http://cvi.asm.org/content/15/7/1095 


Wiederhold NP, Thornton CR, Najvar LK, Kirkpatrick WR, Bocanegra R, Patterson TF. (2009). Comparison of Lateral Flow Technology and Galactomannan and (1->3)-β-D-Glucan assays for Detection of Invasive Pulmonary Aspergillosis. Clinical and Vaccine Immunology 16: 1844-1846.

http://cvi.asm.org/content/16/12/1844


Thornton CR. (2010). Detection of Invasive Aspergillosis. Advances in Applied Microbiology 70: 187-216.

http://www.sciencedirect.com/science/article/pii/S006521641070006X


Hoenigl WM, Koidl C, Duettmann W, Seeber K, Wagner J, Buzina W, Wöfler A, Raggam RB, Thornton CR. (2012). Bronchoalveolar Lavage Lateral-Flow Device Test for Invasive Aspergillosis Diagnosis in Haematological Malignancy and Solid Organ Transplant Patients. Journal of Infection 65: 588-591.

http://www.sciencedirect.com/science/article/pii/S0163445312002897


Wiederhold NP, Najvar LK, Bocanegra R, Kirkpatrick WR, Patterson T, Thornton CR. (2013). Inter-Laboratory and Inter-Study Reproducibility of a Novel Lateral-Flow Device and the Influence of Antifungal Therapy on the Detection of Invasive Pulmonary Aspergillosis. Journal of Clinical Microbiology 51: 459-465.

http://jcm.asm.org/content/51/2/459


White PL, Parr C, Thornton CR, Barnes RA. (2013). An Evaluation of Real-Time PCR, Galactomannan ELISA and a Novel Lateral-Flow Device for Diagnosis of Invasive Aspergillosis. Journal of Clinical Microbiology 51: 1510-1516.

http://jcm.asm.org/content/51/5/1510


Thornton CR. (2013) Lateral Flow Device for Diagnosis of Fungal Infection. Current Fungal Infection Reports 7: 244-251.

http://link.springer.com/article/10.1007%2Fs12281-013-0138-x 


Held J, Schmidt T, Thornton CR, Kotter E, Bertz H. (2013). Comparison of a Novel Aspergillus Lateral-Flow Device and the Platelia® Galactomannan Assay for Diagnosis of Invasive Aspergillosis Following Haematopoietic Stem Cell Transplantation. Infection 41: 1163-1169.

http://link.springer.com/article/10.1007%2Fs15010-013-0472-5


Willinger B, Lackner M, Lass-Florl C, Prattes J, Posch V, Selitsch B, Eschertzhuber S, Hoenigl K, Koidl C, Sereinigg M, Raggam RB, Thornton CR, et al. (2014). Bronchoalveolar Lavage Lateral-Flow Device Test for Invasive Pulmonary Aspergillosis in Solid Organ Transplant Patients: a Semi-Prospective Multicenter Study. Transplantation 98: 898-892.

http://journals.lww.com/transplantjournal/pages/articleviewer.aspx?year=2014&issue=10270&article=00017&type=abstract 


Heonigl M, Prattes J, Spiess B, Wagner J, Prueller F, Raggam RB, Posch V, Duettmann W, Hoenigl K, Wofler A, Buzina W, Reinwald M, Thornton CR, Krause R, Buchheidt D. (2014). Performance of Galactomannan, β-D-glucan, Aspergillus Lateral-Flow Device, Conventional Culture and PCR Tests for Diagnosis of Invasive Pulmonary Aspergillosis in Bronchoalveolar Lavage Fluid. Journal of Clinical Microbiology 52: 2039-2045.

http://jcm.asm.org/content/52/6/2039


Prattes J, Prueller F, Koidl C, Raggam RB, Palfner M, Eigl S, Flick H, Buzina W, Zollner-Schwetz I, Thornton CR, et al. (2014). Novel Tests for Diagnosis of Invasive Pulmonary Aspergillosis in Patients with Underlying Respiratory Diseases. American Journal of Respiratory and Critical Care Medicine 190: 922-929.

http://www.atsjournals.org/doi/abs/10.1164/rccm.201407-1275OC


Thornton CR. (2014). Breaking the Mould – Novel Diagnostic and Therapeutic Strategies for Invasive Pulmonary Aspergillosis in the Immune Deficient Patient. Expert Review of Clinical Immunology 6: 771-780.

http://www.tandfonline.com/doi/full/10.1586/1744666X.2014.904747


Eigl S, Prattes J, Lackner M, Willinger B, Spiess B, Selitsch B, Meilinger M, Reischies F, Neumeister P, Wölfler A, Raggam RB, Flick H, Eschertzhuber S, Krause R, Buchheidt D, Thornton CR, Lass-Flörl C, Hoenigl M. (2015). Multicenter Evaluation of a Lateral-Flow Device Test for Diagnosing Invasive Pulmonary Aspergillosis in ICU Patients. Critical Care 19: 1-9.

http://ccforum.biomedcentral.com/articles/10.1186/s13054-015-0905-x


Eigl S, Prattes J, Reinwald M, Thornton CR, Reischies F, Spiess B, Neumeister P, Zollner-Schwetz I, Raggam RB, Flick H, et al. (2015). Influence of Mould-Active Antifungal Treatment on the Performance of the Aspergillus Specific Bronchoalveolar Lavage Lateral-Flow Device Test. International Journal of Antimicrobial Agents 46: 401-405.

http://www.sciencedirect.com/science/article/pii/S092485791500223X


Prattes J, Lackner M, Eigl S, Reischies F, Raggam RB, Koidl C, Flick H, Wurm R, Palfner M, Wölfler Neumeister P, Thornton CR, Krause R, Lass-Flörl C, Hoenigl M. (2015). Diagnostic Accuracy of the Aspergillus-Specific Bronchoalveolar Lavage Lateral-Flow Assay in Haematological Malignancy Patients. Mycoses 58: 461-469.

http://onlinelibrary.wiley.com/doi/10.1111/myc.12343/abstract;jsessionid=5FC7C915A1CF68135F90B6848531DD0C.f04t03


Miceli M, Goggins MI, Chander P, Sekaran AK, Kizy AE, Samuel L, Jiang H, Thornton CR, Ramesh M, Alangaden G. (2015). Performance of Lateral Flow Device and Galactomannan for the Detection of Aspergillus Species in Bronchoalveolar Lavage Fluid of Patients at Risk for Invasive Pulmonary Aspergillosis. Mycoses 58: 368-374.

http://onlinelibrary.wiley.com/doi/10.1111/myc.12327/abstract